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DoubleDigest Calculator—Thermo Scientific DoubleDigest conveniently calculates the best enzymatic reaction buffer, enzyme concentrations, incubation conditions, and any additives needed in your double digest reaction.
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Otherwise, choose an NEBuffer that results in the most activity for both enzymes. Double Digest Finder. Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product. Choose a DNA, RNA, genome editing, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry. DoubleDigest Calculator—Thermo Scientific DoubleDigest conveniently calculates the best enzymatic reaction buffer, enzyme concentrations, incubation conditions, and any additives needed in your double digest reaction.
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Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang.
Add components to a clean tube in the order shown: 1 µL DNA (concentration 1 µg/µL) 2 µL 10x buffer Aug 22, 2014 Explore NEB's entire suite of interactive online tools at www.neb.com/nebtools. NEBioCalculator™. Double Digest. Finder. Tm. Calculator. Jan 29, 2014 include the popular NEB Tools, Double Digest Finder and Enzyme Finder, as well as NEBuilder®, NEBaseChanger™, and its Tm Calculator. Digesting a DNA substrate with two restriction endonucleases simultaneously ( double digestion) is a common timesaving procedure.
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DoubleDigest conveniently calculates the best enzymatic reaction buffer, enzyme concentrations, incubation conditions, and any additives needed in your double digest reaction. Peak DNA digestion without star activity is best accomplished with conventional Thermo Scientific restriction enzymes using the Five Buffer System . In this video, you will learn how double digests are made even easier with a tool, NEBCloner. NEBCloner provides a nice protocol taking into account buffer, temperature, additive, as well as star activity differences.
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